This policy applies to all studies involving challenge with microorganisms or chemicals that could reasonably be expected to cause significant discomfort for some animals.
Generic protocols for toxicology or infectious disease studied will generally not be considered by the IACUC. Separate protocols for each agent, or at least related groups of agents, must be submitted to the Committee.
If the agent has the potential to cause significant discomfort, the earliest possible endpoint to the study must be defined and used. Refer to Humane Endpoints (in Chapter 13) above. The protocol must justify and state, as clearly as possible, the endpoint to be used. Appendix VII of the Animal Study Protocol Application Form (http://www.research.olemiss.edu/compliance/IACUC/protocol_appforms) must be completed.
Careful consideration must be given to the number of animals required to accomplish the objectives of the study. The number anticipated to be used must be clearly indicated and justified in the protocol. The following website has some helpful tools:
Toxic or lethal challenges with the potential to cause discomfort are not to be given in connection with teaching protocols except on anesthetized animals that will not be allowed to recover consciousness. Use of a very limited number of animals for the purpose of preparing educational materials such as videotapes may be approved.